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| Biphasic Corneal Inflammatory Response during corneal epithelial wound healing in the Mouse |
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| 作者：Zhijie L… 文章来源：Leukocyte Biology Section, Department of Pediatrics and Immunology, Baylor college of Medicine, Houston, TX 77030 点击数1679 更新时间：2004/6/15 文章录入：毛进 责任编辑：毛进 |
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| Objective: To examine the kinetics and mechanisms of inflammatory response
during corneal epithelial wound healing in the mouse. Methods: The central
corneal epithelium (2 mm diameter) was demarcated and removed in both C57Bl/6
wild type and CD18-/- mice. Epithelial healing was analyzed every 6 hours by
image analysis. Neutrophils and macrophages were quantified under
deconvolution microscopy. Enzyme-linked immunosorbent assay (EILSA) was used
to measure IL-1, IL-6, IL-8, MCP-1, MIP-2, and LIX in corneal extracts.
Results: In wild type mice, neutrophil numbers in the corneas exhibited two
distinct peaks at 12 and 30 hours after wounding; macrophages peaked at 24 and
42 hours. In contrast, CD18-/- mice exhibited profoundly low leukocyte
infiltration during the period of the wild type first peak, but elevated
leukocytes at 30 hours (neutrophils) and 36 hours (macrophages) after
wounding. Similarly, all cytokines and chemokines exhibited biphasic and
monophasic patterns in wild type and CD18-/- mice, respectively.
Re-epithelialization was significantly delayed by 6-12 hours in CD18-/- mice.
Conclusions: The corneal inflammatory response to corneal epithelial scraping
is biphasic within 48 hours for both neutrophils and macrophages. The first
wave is apparently CD18 dependent, the second wave CD18 independent. |
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