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Calpains inhibitor PD150606 in the prevention of hydrogen peroxide-induced cataract in vitro |
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| Calpains inhibitor PD150606 in the prevention of hydrogen peroxide-induced cataract in vitro |
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作者：徐雯 文章来源：浙江大学医学院附属二院眼科中心 点击数：1692 更新时间：2005/6/17 10:49:08 
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| Purpose To observe the inhibition of hydrogen peroxide (H2O2)-induced cataract by PD150606 through detection of opacity of lenses, variation of protein and cells, and expression of caspase-3 in lens epithelial cells (LECs). Basic procedures The inhibitory group of rat lenses were cultured in vitro with H2O2 and PD150606, and the positive group with H2O2, the negative group with basic medium only. Photography was taken under microscope in order to detect the opacification; the proportion of water soluble protein (WSP) was determined by Lowry’s method; the number of apoptotic LECs was measured by flow cytometry; the expression of caspase-3 in LECs was determined by Western blot analysis. Main findings There were significant differences of relative gray scale (P<0.05, P<0.05), significant differences of the proportion of WSP (P<0.05 at 6h, 12h and 24h; P<0.01 at 12h and 24h), significant differences of the number of apoptotic LECs (P=0.000, P=0.000) and significant differences of the expression of caspase-3 (P<0.01, P<0.05) at 6h, 12h and 24h between the negative and the positive control group and the inhibitory and the positive control group, respectively. Principle conclusions PD150606——an inhibitor of calpains, can inhibit H2O2-induced opacification and proteolysis of lenses, apoptosis of LECs and the expression of caspase-3 in rat LECs in vitro effectively. The activated calpains can induce degradation of proteins in lenses and apoptosis of LECs, which may play a role in the mechanism of oxidative cataract. Caspase-3-dependent pathway may distribute to apoptosis of LECs induced by calpains. |
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